nsc34 cells (CELLutions Biosystems)
90
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CELLutions Biosystems
nsc34 cells
Nsc34 Cells, supplied by CELLutions Biosystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nsc34+cells/nsc+34+cells/pm39894561-39-0-7
Average 90 stars, based on 1 article reviews
Nsc34 Cells, supplied by CELLutions Biosystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nsc34+cells/nsc+34+cells/pm39894561-39-0-7
Average 90 stars, based on 1 article reviews
nsc34 cells - by Bioz Stars,
2026-09
90/100 stars
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Modification:Article Title: Activated Fibroblast Growth Factor Receptor 1 Mitigated Poly-PR-Induced Oxidative Stress and Protein Translational Impairment. Article Snippet: Plasmids, Cell Culture, and Transfection Expression plasmids pEGFP-1 (Clontech Laboratories Inc., CA, U.S.A.) or pmCherry-N1 (Clontech Laboratories Inc.) harboring PR50 were prepared as described in a previous report.9) pBRPB CAG-mCherry-IP was a gift from Prof. Thomas Tuschl (Addgene plasmid # 106333; http://n2t.net/addgene:106333; RRID: Addgene_106333).33) NSC34-mCherry cells were established by co-transfected with pBRPB CAG-mCherry-IP and the piggyBac transposase into NSC34 cells (Mouse Neuroblastoma × Spinal Cord-34, CELLutions Biosystems, Canada) using Lipofectamine 3000, following the manufacturer’s protocol (Thermo Fisher Scientific Inc., Waltham, MA, U.S.A.). .. Article Title: Neuroprotective effects of activated fibroblast growth factor receptor 1 via the suppression of p53 accumulation against poly-PR-mediated toxicity. Article Snippet: A GGGGCC hexanucleotide repeat expansion (HRE) within the C9orf72 gene is a major causative factor in amyotrophic lateral sclerosis (ALS).. This aberrant HRE results in the generation of five distinct dipeptide repeat proteins (DPRs).. Among the DPRs, poly-PR accumulates in the nucleus and exhibits particularly strong toxicity to motor and cortical neurons. Cell Culture:Article Title: Protrusive waves guide 3D cell migration along nanofibers Article Snippet: .. NIH-3T3 fibroblasts (ATCC), immortalized mouse embryonic fibroblasts RPTPα , REF52 fibroblasts (ATCC), HeLA cells (ATCC), HEK-293 cells (ATCC), Article Title: Small Hexokinase 1 Peptide against Toxic SOD1 G93A Mitochondrial Accumulation in ALS Rescues the ATP-Related Respiration Article Snippet: Cells were cultured in 5% CO 2 in DMEM/F12 (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% tetracycline-free FBS (GIBCO, Waltham, MA, USA), penicillin/streptomycin antibiotic and 200 μg/mL G418 (Carlo Erba, Milan, Italy) for selection maintenance. .. The maximal expression of SOD1 proteins was achieved by the addition of 2 μg/mL doxycycline (Sigma-Aldrich) to the medium after 48 h. The parental Expressing:Article Title: Small Hexokinase 1 Peptide against Toxic SOD1 G93A Mitochondrial Accumulation in ALS Rescues the ATP-Related Respiration Article Snippet: Cells were cultured in 5% CO 2 in DMEM/F12 (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% tetracycline-free FBS (GIBCO, Waltham, MA, USA), penicillin/streptomycin antibiotic and 200 μg/mL G418 (Carlo Erba, Milan, Italy) for selection maintenance. .. The maximal expression of SOD1 proteins was achieved by the addition of 2 μg/mL doxycycline (Sigma-Aldrich) to the medium after 48 h. The parental Control:Article Title: Small Hexokinase 1 Peptide against Toxic SOD1 G93A Mitochondrial Accumulation in ALS Rescues the ATP-Related Respiration Article Snippet: Cells were cultured in 5% CO 2 in DMEM/F12 (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% tetracycline-free FBS (GIBCO, Waltham, MA, USA), penicillin/streptomycin antibiotic and 200 μg/mL G418 (Carlo Erba, Milan, Italy) for selection maintenance. .. The maximal expression of SOD1 proteins was achieved by the addition of 2 μg/mL doxycycline (Sigma-Aldrich) to the medium after 48 h. The parental |
![PTEN -siRNA nanoparticle transfection enables efficient gene silencing with minimal impact on neuron health (A) PTEN -siRNA nanoparticles (100 nM) demonstrated highly efficient knockdown of target PTEN mRNA in differentiated <t>NSC34</t> neurons, with peak knockdown on Day 3 for the delivered siRNA [t(2) = −4.840, p = 0.02007; one-tailed] with expression levels returning to baseline by Day 7 [t(2) = 0.05373, p = 0.5190; one-tailed]. RNA fold change and associated error was assessed using the 2 −ΔΔCt method and normalized to GAPDH as per (B) Treatment of neurons with PTEN- siRNA nanoparticles at 100 nM had a non-significant effect on the metabolic activity at 3 [t(2) = 0.6370, p = 0.5893, 95 % CI 74.4820<x <134.3874] and 7 days [t(2) = −3.390, p = 0.07708, 95 % CI -66.21<x<104.0] post-transfection. (C – F) Transfection of PTEN -siRNA nanoparticles does not affect neuronal morphology. Control and PTEN -siRNA nanoparticle treated neurons have a similar distribution, density, and morphology compared to control neurons (C, D). Higher magnification images showed that all neurons (control and treated) possessed a typical neuronal morphology with several neurites extending from each cell body (E, F). All cells were stained for F-actin (green = phalloidin) and nuclei (blue = DAPI). Scale Bar in (C, D): 100 μm; Scale Bar in (E, F): 50 μm. Significance code: ns = p > 0.1; • = p ≤ 0.1, ∗ = p ≤ 0.05, ∗∗ = p ≤ 0.01, ∗∗∗ = p ≤ 0.001, ∗∗∗∗ = p ≤ 0.0001.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8063/pmc12908063/pmc12908063__gr1.jpg)
![Scaffold-mediated siRNA delivery does not affect the viability of neurons, results in prolonged downregulation of PTEN, and stimulates increased expression of BCL2 and GAP43 mRNA . (A) The metabolic activity of NSC34 neurons cultured on PTEN -siRNA activated scaffolds peaked by day 3 post-transfection [t(2) = 4.0599, p = 0.05565, 95 % CI 93.954<x<308.3], decreased by day 7 [t(2) = −15.068, p = 0.004375, 95 % CI 49.7157<x<72.05] and was similar to control levels by day 21 [t(2) = −0.1042, p = 0.9265, 95 % CI 83.23<x<116.0]. Metabolic activity normalization was performed relative to the untreated cells. (B) LDH assay revealed a consistent but small increase in cell stress over a span of 21 days. (C – D) Analysis of PTEN gene expression in scaffold transfected neurons over 21 days showed a significant decrease in expression levels [t(4) = −3.2927, p = 0.01507; one-tailed] 3 days after transfection that was then followed by a gradual return toward untreated control levels denoted by the blue regression line which approaches the red untreated control line after 21 days. (E – F) The change in BCL2 expression 3-, 7-, and 21-days post-transfection showed a significant initial elevation [t(4) = 2.092, p = 0.05227, one-tailed], followed by a gradual reduction converging toward untreated control levels. (G – H). Similarly, GAP43 expression was characterised by of an initial significant rise [t(4) = 2.1748, p = 0.04765, one-tailed] in levels that was followed by a decrease over the 21-day culture period. Significance code: ns = p > 0.1; • = p ≤ 0.1, ∗ = p ≤ 0.05, ∗∗ = p ≤ 0.01, ∗∗∗ = p ≤ 0.001, ∗∗∗∗ = p ≤ 0.0001.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8063/pmc12908063/pmc12908063__gr4.jpg)